Annexin VI Polyklonaler Antikörper

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• KD/KO Validated

Annexin VI Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12542-1-PBS

Synonyme

Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6

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  WB analysis using 12542-1-AP (same clone as 12542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12542-1-AP (same clone as 12542-1-PBS)

  WB result of Annexin VI antibody (12542-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin VI transfected HeLa cells. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12542-1-AP (same clone as 12542-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12542-1-AP (same clone as 12542-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12542-1-AP (same clone as 12542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse placenta using 12542-1-AP (same clone as 12542-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12542-1-AP (same clone as 12542-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12542-1-AP (same clone as 12542-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12542-1-AP (same clone as 12542-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12542-1-AP (same clone as 12542-1-PBS)

  IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12542-1-AP (same clone as 12542-1-PBS)

  IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with mouse kidney tissue lysate 3400 ug. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12542-1-AP (Annexin VI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12542-1-AP (same clone as 12542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12542-1-AP (same clone as 12542-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12542-1-AP (Annexin VI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12542-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Annexin VI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Annexin VI fusion protein Ag3263
• Vollständiger Name: annexin A6
• Berechnetes Molekulargewicht: 673 aa, 76 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC017046
• Gene symbol: Annexin VI
• Gene ID (NCBI): 309
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.