Apelin Polyklonaler Antikörper

Apelin Polyklonal Antikörper für IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11497-1-PBS

Synonyme

APLN, APEL, APJ endogenous ligand

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  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11497-1-AP (same clone as 11497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11497-1-PBS bindet in IHC, IF/ICC, IF-P, Indirect ELISA Apelin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Apelin fusion protein Ag2047
• Vollständiger Name: apelin
• Berechnetes Molekulargewicht: 77 aa, 9 kDa
• GenBank-Zugangsnummer: BC021104
• Gene symbol: Apelin
• Gene ID (NCBI): 8862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apelin, isolated from bovine stomach tissue extractsis, is recognized as the endogenous ligand of the angiotensin-like-receptor 1 (APJ), and the human orphan G-protein-coupled receptor. Apelin is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin has been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Apelin is also known to be involved in the maintenance of INS sensitivity and play an important role in liver disease.