Apoe Rekombinanter Antikörper

Apoe Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240786C3

Kat-Nr. : 83728-4-PBS

Synonyme

apolipoprotein E, AI255918, 240786C3

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  WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 83728-4-RR (same clone as 83728-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apoe antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83728-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

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Geprüfte Anwendungen

Produktinformation

83728-4-PBS bindet in WB, IHC, IF/ICC, ELISA Apoe und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: Apolipoprotein E
• Berechnetes Molekulargewicht: 36kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_001305819.1
• Gene symbol: Apoe
• Gene ID (NCBI): 11816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).