Apoe Rekombinanter Antikörper

Apoe Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240786C3

Kat-Nr. : 83728-4-RR

Synonyme

apolipoprotein E, AI255918, 240786C3

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  WB analysis using 83728-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83728-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83728-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83728-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 83728-4-RR

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 83728-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 83728-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apoe antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83728-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mauslebergewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83728-4-RR bindet in WB, IHC, IF/ICC, ELISA Apoe und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: Apolipoprotein E
• Berechnetes Molekulargewicht: 36kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_001305819.1
• Gene symbol: Apoe
• Gene ID (NCBI): 11816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).