Arginase-1 Monoklonaler Antikörper

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• KD/KO Validated

Arginase-1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

5D6D12

Kat-Nr. : 66129-1-PBS

Synonyme

ARG1, Arginase 1, liver Arginase, 5D6D12, ARG 1

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  WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66129-1-Ig (Arginase-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  Sample test of MP51326-2

  Sample test of MP51326-2, Arginase-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66129-2-PBS. Detection antibody: 66129-1-PBS.

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  IP experiment of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  IP result of anti-Arginase-1 (IP:66129-1-Ig, 4ug; Detection:66129-1-Ig 1:10000) with mouse liver tissue lysate 2240 ug. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51326-2

  Cytometric bead array standard curve of MP51326-2, Arginase-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66129-2-PBS. Detection antibody: 66129-1-PBS. Standard:Ag8595. Range: 0.195-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66129-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA, Sample test Arginase-1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Arginase-1 fusion protein Ag8810
• Vollständiger Name: arginase, liver
• Berechnetes Molekulargewicht: 236aa,25 kDa; 322aa,35 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC005321
• Gene symbol: Arginase-1
• Gene ID (NCBI): 383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).