Arp5/ACTR5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Arp5/ACTR5 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21505-1-PBS

Synonyme

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  WB analysis of mouse cerebellum using 21505-1-AP (same clone as 21505-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21505-1-AP (same clone as 21505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 21505-1-AP (same clone as 21505-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 21505-1-AP (same clone as 21505-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 21505-1-AP (same clone as 21505-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 21505-1-AP (same clone as 21505-1-PBS)

  Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21505-1-AP (same clone as 21505-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ACTR5 antibody 21505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21505-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA Arp5/ACTR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Arp5/ACTR5 fusion protein Ag15976
• Vollständiger Name: ARP5 actin-related protein 5 homolog (yeast)
• Berechnetes Molekulargewicht: 607 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC038402
• Gene symbol: ARP5/ACTR5
• Gene ID (NCBI): 79913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)