Arrestin C Rekombinanter Antikörper

Arrestin C Rekombinant Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242751B12

Kat-Nr. : 85067-5-PBS

Synonyme

Arrestin-C, ARR3, C-arrestin, CAR, ARRX

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  WB analysis using 85067-5-RR (same clone as 85067-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85067-5-RR (ARR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85067-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 85067-5-RR (same clone as 85067-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85067-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 85067-5-RR (same clone as 85067-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85067-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85067-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85067-5-RR against Human Arrestin C were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85067-5-PBS bindet in WB, IF-P, Indirect ELISA Arrestin C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Arrestin C fusion protein Ag1580
• Vollständiger Name: arrestin 3, retinal (X-arrestin)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC012096
• Gene symbol: Arrestin C
• Gene ID (NCBI): 407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.