Arrestin C Rekombinanter Antikörper

Arrestin C Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

242751B12

Kat-Nr. : 85067-5-RR

Synonyme

Arrestin-C, ARR3, C-arrestin, CAR, ARRX

---

• 

  WB analysis using 85067-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85067-5-RR (ARR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse eye using 85067-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 85067-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85067-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85067-5-RR against Human Arrestin C were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, rat eye tissues
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

85067-5-RR bindet in WB, IF-P, ELISA Arrestin C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Arrestin C fusion protein Ag1580
• Vollständiger Name: arrestin 3, retinal (X-arrestin)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC012096
• Gene symbol: Arrestin C
• Gene ID (NCBI): 407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.

Publikationen

Species	Application	Title
human	IF	Sci Rep; The role of HMOX1-mediated ferroptosis in blue light-induced damage to retinal pigment epithelium; Authors - Chunyi Ji