Ataxin 2 Monoklonaler Antikörper

Ataxin 2 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E8A2

Kat-Nr. : 68316-1-PBS

Synonyme

ATXN2, 1E8A2, ataxin2, Ataxin-2, ATX2

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  WB analysis using 68316-1-Ig (same clone as 68316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68316-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68316-1-Ig (same clone as 68316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68316-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68316-1-Ig (same clone as 68316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68316-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 68316-1-Ig (same clone as 68316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68316-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 68316-1-Ig (same clone as 68316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68316-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68316-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Ataxin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ataxin 2 fusion protein Ag17402
• Vollständiger Name: ataxin 2
• Berechnetes Molekulargewicht: 1313 aa, 140 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC114546
• Gene symbol: ATXN2
• Gene ID (NCBI): 6311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]