Ataxin 2 Rekombinanter Antikörper

Ataxin 2 Rekombinant Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250888E4

Kat-Nr. : 80919-2-RR

Synonyme

ATXN2, ataxin2, Ataxin-2, ATX2, ATXN 2

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  WB analysis using 80919-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80919-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 80919-2-RR

  IP result of anti-Ataxin 2 (IP:80919-2-RR, 4ug; Detection:80919-2-RR 1:1000) with NIH/3T3 cells lysate 1200 ug.

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  IHC staining of mouse brain using 80919-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80919-2-RR (Ataxin 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 80919-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Ataxin 2 antibody (80919-2-RR, Clone: 250888E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80919-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80919-2-RR against Human Ataxin 2 were performed. The affinity constant is 0.153 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80919-2-RR bindet in WB, IHC, IF-P, IP, ELISA Ataxin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ataxin 2 fusion protein Ag16470
• Vollständiger Name: ataxin 2
• Berechnetes Molekulargewicht: 1313 aa, 140 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC114546
• Gene symbol: ATXN2
• Gene ID (NCBI): 6311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]