Atox1 Rekombinanter Antikörper

Atox1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241566A7

Kat-Nr. : 84305-1-PBS

Synonyme

241566A7, Atx1, HAH1

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  WB analysis using 84305-1-RR (same clone as 84305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84305-1-RR (Atox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01193-1

  Sandwich ELISA standard curve of MP01193-1, Mouse Atox1 Recombinant Matched Antibody Pair - PBS only. 84305-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35290. 84305-2-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

• 

  Sample test of MP01193-1

  The mean Atox1 concentration was determined to be 89.29 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

• 

  IHC staining of mouse heart using 84305-1-RR (same clone as 84305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84305-1-RR (Atox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01193-1

  Cytometric bead array standard curve of MP01193-1, MOUSE Atox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84305-1-PBS. Detection antibody: 84305-2-PBS. Standard: Ag35290. Range: 0.313-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84305-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Atox1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Atox1 fusion protein Ag35290
• Vollständiger Name: ATX1 (antioxidant protein 1) homolog 1 (yeast)
• Berechnetes Molekulargewicht: 7 kDa
• Beobachtetes Molekulargewicht: 7 kDa
• GenBank-Zugangsnummer: NM_009720
• Gene symbol: Atox1
• Gene ID (NCBI): 11927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.