ATP1B1 Rekombinanter Antikörper

ATP1B1 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

243093E3

Kat-Nr. : 85916-1-PBS

Synonyme

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

---

• 

  WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02193-3

  Sandwich ELISA standard curve of MP02193-3, Mouse ATP1B1 Recombinant Matched Antibody Pair - PBS only. 85916-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3007. 85916-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

• 

  Sample test of MP02193-3

  The mean ATP1B1 concentration was determined to be 58,169.6 pg/mL in mouse heart extract based on a 6.9 mg/mL extract load.

• 

  IHC staining of mouse liver using 85916-1-RR (same clone as 85916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85916-1-RR (same clone as 85916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

85916-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test ATP1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP1B1 fusion protein Eg3007
• Vollständiger Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_009721.6
• Gene symbol: Atp1b1
• Gene ID (NCBI): 11931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)