B23/NPM1 Monoklonaler Antikörper

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• KD/KO Validated

B23/NPM1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F12A3

Kat-Nr. : 60096-1-PBS

Synonyme

B23, NPM1, 4F12A3, NPM, Nucleophosmin

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  WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

  WB result of B23/NPM1 antibody (60096-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B23/NPM1 transfected HepG2 cells. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60096-1-Ig (same clone as 60096-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60096-1-Ig (same clone as 60096-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60096-1-Ig (same clone as 60096-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60096-1-Ig (same clone as 60096-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60096-1-Ig (same clone as 60096-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60096-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA B23/NPM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B23/NPM1 fusion protein Ag7415
• Vollständiger Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC002398
• Gene symbol: NPM1
• Gene ID (NCBI): 4869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.