B23/NPM1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

B23/NPM1 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

4F12A3

Kat-Nr. : CL594-60096

Synonyme

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

---

• 

  IF Staining of HepG2 using CL594-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using the CoraLite®594-conjugated version of this antibody, CL594-60096 (B23/NPM1 antibody), at dilution of 1:100. F-actin is stained using CL488-phalloidin (Green) and DNA is stained by DAPI (blue).

• 

  IF Staining of HepG2 using CL594-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-60096 (B23/NPM1 antibody) at dilution of 1:100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-60096 bindet in IF/ICC B23/NPM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B23/NPM1 fusion protein Ag7415
• Vollständiger Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC002398
• Gene symbol: NPM1
• Gene ID (NCBI): 4869
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publikationen

Species	Application	Title
	IF	J Hematol Oncol; METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation; Authors - Meilin Xue