Beta-2-Microglobulin Monoklonaler Antikörper

Beta-2-Microglobulin Monoklonal Antikörper für IF/ICC, FC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3F4

Kat-Nr. : 68395-1-Ig

Synonyme

B2M, 1H3F4, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

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  IF Staining of NCCIT using 68395-1-Ig

  Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 68395-1-Ig

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 68395-1-Ig

  1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NCCIT-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68395-1-Ig bindet in IF/ICC, FC, ELISA Beta-2-Microglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta-2-Microglobulin fusion protein Eg31815
• Vollständiger Name: beta-2-microglobulin
• Berechnetes Molekulargewicht: 119 aa, 14 kDa
• Beobachtetes Molekulargewicht: 12-14 kDa
• GenBank-Zugangsnummer: BC032589
• Gene symbol: B2M
• Gene ID (NCBI): 567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.