Beta-2-Microglobulin Monoklonaler Antikörper

Beta-2-Microglobulin Monoklonal Antikörper für Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

4D4D5

Kat-Nr. : 68395-3-PBS

Synonyme

B2M, 4D4D5, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2-Microglobulin

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  Sample test of MP50108-1

  Cytometric bead array of A375 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of A375 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of HeLa cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of HeLa cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of U-251 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of U-251 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LNCaP, THP-1, Karpas299, BT-549, HT-29, U266B1, Kasumi-1, Jurkat, HCT116, TT, MCF-7, K-562, Raji, NCCIT, BxPC-3, PC-3, Ramos, A549, SK-N-SH, U-251, HepG2, Caco-2, 293, 293T, hTERT-RPE1, A431, HT-1080, Saos-2, SH-SY5Y, UC-MSCS. Complete culture medium was served as blank control.

• 

  Sample test of MP50108-1

  Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: hTERT-RPE1, U251, A431, PC-12, ROS1728, NIH/3T3, mMSCS. Complete culture containing DMEM and FBS was served as blank control. Note that this matched antibody pair recognizes human B2M (beta 2 macroglobulin) but does not recognize rat or mouse B2M.

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  Cytometric bead array standard curve of MP50108-1

  Cytometric bead array standard curve of MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Standard:Eg31815. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68395-3-PBS bindet in Cytometric bead array, Indirect ELISA, Sample test Beta-2-Microglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: beta-2-microglobulin
• Berechnetes Molekulargewicht: 119 aa, 14 kDa
• GenBank-Zugangsnummer: BC032589
• Gene symbol: B2M
• Gene ID (NCBI): 567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.