CD57 Polyklonaler Antikörper

CD57 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 19401-1-AP

Synonyme

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

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Galerie der Validierungsdaten

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  WB analysis using 19401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19401-1-AP (CD57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Prostatakarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

19401-1-AP bindet in WB, IHC, IF, ELISA CD57 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD57 fusion protein Ag13550
• Vollständiger Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 70 kDa
• GenBank-Zugangsnummer: BC010466
• Gene symbol: B3GAT1
• Gene ID (NCBI): 27087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.; Authors - Sihui Yu
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	IF	Front Bioeng Biotechnol; Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.; Authors - Jie Chen
human	IHC	BMC Med Genomics; Establishment and validation of an immune infiltration predictive model for ovarian cancer; Authors - Zhenxia Song
human,mouse	IHC	Aging (Albany NY); Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma; Authors - Xiaonan Zhang