B3GAT1 Monoklonaler Antikörper

B3GAT1 Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1F4A11

Kat-Nr. : 66711-1-Ig

Synonyme

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

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  WB analysis using 66711-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66711-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66711-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Maushirngewebe, PC-3-Zellen, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66711-1-Ig bindet in WB, FC (Intra), ELISA B3GAT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B3GAT1 fusion protein Ag13593
• Vollständiger Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 100 kDa
• GenBank-Zugangsnummer: BC010466
• Gene symbol: B3GAT1
• Gene ID (NCBI): 27087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.