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B3GNT2 Polyklonaler Antikörper

B3GNT2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14200-1-PBS

Synonyme

B3GALT7, b3Gal-T7, B3GNT1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7

Filter:
  • Western Blot (WB) analysis of various lysates using B3GNT2 Polyclonal antibody (14200-1-AP)

    WB analysis using 14200-1-AP (same clone as 14200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using B3GNT2 Polyclonal antibody (14200-1-AP)

    IHC staining of mouse testis using 14200-1-AP (same clone as 14200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14200-1-AP (B3GNT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14200-1-PBS bindet in WB, IHC, Indirect ELISA B3GNT2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen B3GNT2 fusion protein Ag5404
Vollständiger Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Berechnetes Molekulargewicht 9 kDa
Beobachtetes Molekulargewicht69 kDa
GenBank-ZugangsnummerBC030579
Gene symbol B3GNT2
Gene ID (NCBI) 10678
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.