B3GNT3 Polyklonaler Antikörper

B3GNT3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 18098-1-AP

Synonyme

Beta-1,3-N-acetylglucosaminyltransferase 3, Beta-1,3-Gn-T3, Beta-1,3-GalTase 8, Beta-1,3-galactosyltransferase 8, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase

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  WB analysis using 18098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18098-1-AP (B3GNT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18098-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18098-1-AP (B3GNT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18098-1-AP (B3GNT3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 18098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18098-1-AP (B3GNT3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 18098-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using B3GNT3 antibody (18098-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslungengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 6 publications below

Produktinformation

18098-1-AP bindet in WB, IHC, IF/ICC, ELISA B3GNT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B3GNT3 fusion protein Ag12061
• Vollständiger Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
• Berechnetes Molekulargewicht: 372 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC066876
• Gene symbol: B3GNT3
• Gene ID (NCBI): 10331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) is a type II transmembrane protein located in the Golgi apparatus and contributes to the regulation of L-selectin ligand function, lymphocyte trafficking, and T cell homing. B3GNT3 is overexpressed in breast cancer, and also plays a critical role in the aggressiveness of pancreatic cancer (PMID: 33718036, 30466404). Western blot analysis detected B3GNT3 at an apparent molecular mass of 43 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1.; Authors - Chia-Wei Li
human	IHC	Transl Lung Cancer Res; Identifying the prognostic significance of B3GNT3 with PD-L1 expression in lung adenocarcinoma.; Authors - Xuefeng Leng
human	WB,IHC	PLoS One; B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.; Authors - Weijing Zhang
human	WB	BMC Bioinformatics; A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma; Authors - Jin-Xiao Liang
human	WB,IHC	J Clin Pathol; B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.; Authors - Liuwei Gao
human	IHC	Oncol Lett; B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.; Authors - Kaiwen Kong