B3GNT6 Polyklonaler Antikörper

B3GNT6 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21291-1-PBS

Synonyme

BGnT-6, BGnT 6, Beta-1,3-N-acetylglucosaminyltransferase 6, B3Gn-T6, B3Gn T6

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  WB analysis using 21291-1-AP (same clone as 21291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21291-1-AP (B3GNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21291-1-PBS bindet in WB, IHC, Indirect ELISA B3GNT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B3GNT6 fusion protein Ag15825
• Vollständiger Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• Berechnetes Molekulargewicht: 384 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC103910
• Gene symbol: B3GNT6
• Gene ID (NCBI): 192134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)