B3GNT7 Polyklonaler Antikörper

B3GNT7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22879-1-AP

Synonyme

BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7

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  WB analysis of Jurkat using 22879-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22879-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 22879-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22879-1-AP bindet in WB, IHC, ELISA B3GNT7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B3GNT7 fusion protein Ag18890
• Vollständiger Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
• Berechnetes Molekulargewicht: 330 aa, 37 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC148680
• Gene symbol: B3GNT7
• Gene ID (NCBI): 93010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).