B4GALT3 Polyklonaler Antikörper

B4GALT3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11041-1-PBS

Synonyme

b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3

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  WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B4GALT3 antibody (11041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11041-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA B4GALT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B4GALT3 fusion protein Ag1487
• Vollständiger Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC009985
• Gene symbol: B4GALT3
• Gene ID (NCBI): 8703
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).