B4GALT6 Polyklonaler Antikörper

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• KD/KO Validated

B4GALT6 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20148-1-PBS

Synonyme

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  WB analysis of HEK-293 using 20148-1-AP (same clone as 20148-1-PBS)

  WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 20148-1-AP (same clone as 20148-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20148-1-AP (same clone as 20148-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20148-1-AP (same clone as 20148-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20148-1-AP (same clone as 20148-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20148-1-AP (same clone as 20148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20148-1-AP (same clone as 20148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20148-1-AP (same clone as 20148-1-PBS)

  Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20148-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA B4GALT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B4GALT6 fusion protein Ag13686
• Vollständiger Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• Berechnetes Molekulargewicht: 382 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC074884
• Gene symbol: B4GALT6
• Gene ID (NCBI): 9331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).