CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(73)

CloneNo.

1E2F10

Kat-Nr. : 66406-1-Ig

Synonyme

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

---

• 

  WB analysis of Raji using 66406-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DC2.4 using 66406-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66406-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, DC2.4-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IF: See 24 publications below

Produktinformation

66406-1-Ig bindet in WB, IF, ELISA CD80 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD80 fusion protein Ag5615
• Vollständiger Name: CD80 molecule
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC042665
• Gene symbol: CD80
• Gene ID (NCBI): 941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publikationen

Species	Application	Title
mouse		Acta Pharm Sin B; Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy; Authors - Lina Guo
human	IF	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
mouse	WB	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
		J Nanobiotechnology; Dual-modality immune nano-activator harnessing Mn2⁺ and quercetin to potentiate the cGAS-STING pathway for advanced cancer metalloimmunotherapy; Authors - Shanshan Ma
mouse,human		Theranostics; ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.; Authors - Xiaozheng Chen
human	WB	Int J Biol Macromol; Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells; Authors - Xianglong Liu