CD86 Polyklonaler Antikörper

CD86 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 30691-1-AP

Synonyme

B7, B7 2, B7.2, B70, B7-2

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  WB analysis using 30691-1-AP

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DC2.4-Zellen, Mausmilzgewebe, Rattenmilzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Produktinformation

30691-1-AP bindet in WB, IHC, IF, ELISA CD86 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD86 fusion protein Ag33514
• Vollständiger Name: CD86 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_019388
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publikationen

Species	Application	Title
mouse	IF	J Inflamm Res; B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue; Authors - Peizhi Lu
mouse	WB	J Control Release; Reprogramming tumor-associated macrophages by a dually targeted milk exosome system as a potent monotherapy for cancer; Authors - Ying Chen
rat,mouse	WB,IF	Brain Res Bull; Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization; Authors - Qiannan Zhang
mouse	WB	Adv Healthc Mater; Legumain-Guided Ferulate-Peptide Self-Assembly Enhances Macrophage-Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction; Authors - Delong Xu
mouse	WB	Inflammation; Protective Effects of a Dihydrodiazepine Against Endotoxin Shock Through Suppression of TLR4/NF-κB/IRF3 Signaling Pathways; Authors - Hamza Hanieh
rat,mouse	IHC,IF	Turk J Med Sci; Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats; Authors - Bo Ke