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CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250726D3

Kat-Nr. : 86252-1-PBS

Synonyme

B7 2, B7-2, CD86 molecule

Filter:
  • Western Blot (WB) analysis of rat spleen tissue using CD86 Recombinant antibody (86252-1-RR)

    WB analysis of rat spleen using 86252-1-RR (same clone as 86252-1-PBS)

    rat spleen tissue was subjected to SDS PAGE followed by western blot with 86252-1-RR (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86252-1-PBS in a different storage buffer formulation.

  • Sample test of MP02379-1

    Sample test of MP02379-1

    The mean CD86 concentration was determined to be 105.1 ng/mL in Rat spleen tissue extract based on a 8.8 mg/mL extract load.

  • Cytometric bead array standard curve of MP02379-1

    Cytometric bead array standard curve of MP02379-1

    Cytometric bead array standard curve of MP02379-1, RAT CD86 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86252-2-PBS. Detection antibody: 86252-1-PBS. Standard: Eg3586. Range: 0.312-40 ng/mL.

Geprüfte Anwendungen

Produktinformation

86252-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA, Sample test CD86 und zeigt Reaktivität mit Ratten

Getestete Reaktivität Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD86 fusion protein Eg3586
Vollständiger Name CD86 molecule
Berechnetes Molekulargewicht36 kDa
Beobachtetes Molekulargewicht70-75 kDa
GenBank-ZugangsnummerNM_020081.1
Gene symbol Cd86
Gene ID (NCBI) 56822
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)