ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240243G5

Kat-Nr. : 83375-2-PBS

Synonyme

B7-H2/ICOSLG, B7-H2, CD275, Icosl, 240243G5

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  WB analysis using 83375-2-RR (same clone as 83375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83375-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 83375-2-RR (same clone as 83375-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83375-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83375-2-RR (same clone as 83375-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83375-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00376-4

  Sandwich ELISA standard curve of MP00376-4, Mouse ICOSLG/B7-H2/CD275 Monoclonal Matched Antibody Pair - PBS only. 83375-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0923. 83375-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

• 

  Sample test of MP00376-4

  Serum of sixteen individual healthy mices were measured. The mouse ICOSLG/B7-H2/CD275 concentration of detected samples was determined to be 6.7 ng/mL with a range of 4.7 - 10.5 ng/mL.

• 

  Sample test of MP00376-4

  J774A.1 mouse reticulum cell sarcoma macrophage cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse ICOSLG/B7-H2/CD275 and measured 692.8 pg/mL.

• 

  Affinity and Kinetic Characterization of 83375-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83375-2-RR against Mouse B7-H2/ICOSLG were performed. The affinity constant is 0.292 nM.

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Geprüfte Anwendungen

Produktinformation

83375-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Eg0923
• Vollständiger Name: icos ligand
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_015790.3
• Gene symbol: CD275
• Gene ID (NCBI): 50723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.