ICOSLG/B7-H2/CD275 Rekombinanter Antikörper

ICOSLG/B7-H2/CD275 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240243G5

Kat-Nr. : 83375-2-RR

Synonyme

B7-H2/ICOSLG, B7-H2, CD275, Icosl, GL50

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  WB analysis using 83375-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 83375-2-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83375-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83375-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83375-2-RR against Mouse B7-H2/ICOSLG were performed. The affinity constant is 0.292 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushirngewebe, Mausherzgewebe, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83375-2-RR bindet in WB, ELISA ICOSLG/B7-H2/CD275 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: icos ligand
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: NM_015790.3
• Gene symbol: CD275
• Gene ID (NCBI): 50723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).