B7-H3/CD276 Polyklonaler Antikörper

B7-H3/CD276 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30052-1-AP

Synonyme

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

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  WB analysis using 30052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HuH-7 using 30052-1-AP

  IP result of anti-B7-H3/CD276 (IP:30052-1-AP, 4ug; Detection:30052-1-AP 1:15000) with HuH-7 cells lysate 840 ug.

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  IHC staining of human lung cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 30052-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 30052-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using B7-H3/CD276 antibody (30052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, U2OS-Zellen
• Erfolgreiche IP: HuH-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

30052-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA B7-H3/CD276 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD276 molecule
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: NM_001024736
• Gene symbol: CD276
• Gene ID (NCBI): 80381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Publikationen

Species	Application	Title
human	WB	Oncol Res; Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3; Authors - Yan Lu