B7‑H4 Polyklonaler Antikörper

B7‑H4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12080-1-PBS

Synonyme

VTCN1, B7S1, B7-H4, B7H4, B7h.5

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  WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12080-1-AP (same clone as 12080-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 12080-1-AP (same clone as 12080-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12080-1-PBS bindet in WB, IHC, Indirect ELISA B7‑H4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B7‑H4 fusion protein Ag2712
• Vollständiger Name: V-set domain containing T cell activation inhibitor 1
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC065717
• Gene symbol: B7-H4
• Gene ID (NCBI): 79679
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.