B7-H4 Rekombinanter Antikörper

B7-H4 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250392C3

Kat-Nr. : 86484-1-PBS

Synonyme

B7H4, B7S1, B7x, V-set domain-containing T-cell activation inhibitor 1, Vtcn1

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  WB analysis using 86484-1-RR (same clone as 86484-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86484-1-RR (B7-H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86484-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02632-1

  The mean B7-H4 concentration was determined to be 23.71 ng/mL in rat liver extract based on a 8.20 mg/mL extract load and 16.72 ng/mL in mouse uterus extract based on a 3.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02632-1

  Sandwich ELISA standard curve of MP02632-1, Rat B7-H4 Recombinant Matched Antibody Pair - PBS only. 86484-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3230. 86484-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86484-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA B7-H4 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: B7-H4 fusion protein Eg3230
• Vollständiger Name: V-set domain containing T cell activation inhibitor 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_001024244.1
• Gene symbol: Vtcn1
• Gene ID (NCBI): 295322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.