B7-H6 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

B7-H6 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240808F7

Kat-Nr. : 83908-3-RR

Synonyme

B7H6, NCR3LG1, 240808F7, B7 H6, B7 homolog 6

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  WB analysis of HEK-293 using 83908-3-RR

  WB result of B7-H6 antibody (83908-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H6 transfected HEK-293 cells.

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  WB analysis using 83908-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83908-3-RR (B7H6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83908-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83908-3-RR against Human B7-H6 were performed. The affinity constant is 2.38 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83908-3-RR bindet in WB, ELISA B7-H6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: B7-H6 fusion protein Eg0007
• Vollständiger Name: hypothetical protein DKFZp686O24166
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC136800
• Gene symbol: B7H6
• Gene ID (NCBI): 374383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).