BAALC Polyklonaler Antikörper

BAALC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24997-1-PBS

Synonyme

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  WB analysis of HEK-293 using 24997-1-AP (same clone as 24997-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 24997-1-AP (same clone as 24997-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)

  IP result of anti-BAALC (IP:24997-1-AP, 4ug; Detection:24997-1-AP 1:300) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24997-1-AP (same clone as 24997-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24997-1-AP (BAALC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24997-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA BAALC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAALC fusion protein Ag21850
• Vollständiger Name: brain and acute leukemia, cytoplasmic
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19-25 kDa
• GenBank-Zugangsnummer: BC011517
• Gene symbol: BAALC
• Gene ID (NCBI): 79870
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.