BACE1 Polyklonaler Antikörper

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BACE1 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12807-1-PBS

Synonyme

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  WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12807-1-AP (same clone as 12807-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 12807-1-AP (same clone as 12807-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12807-1-AP (same clone as 12807-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 12807-1-AP (same clone as 12807-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12807-1-AP (same clone as 12807-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IP experiment using 12807-1-AP (same clone as 12807-1-PBS)

  IP result of BACE1 antibody (12807-1-AP for IP and Detection) with mouse brain tissue lysate. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12807-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA BACE1 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BACE1 fusion protein Ag3559
• Vollständiger Name: beta-site APP-cleaving enzyme 1
• Berechnetes Molekulargewicht: 501 aa, 56 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa, 42-56 kDa
• GenBank-Zugangsnummer: BC036084
• Gene symbol: BACE1
• Gene ID (NCBI): 23621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).