BACH1 Rekombinanter Antikörper

BACH1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

4K14

Kat-Nr. : 82842-7-PBS

Synonyme

4K14, BACH 1, BACH-1

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  WB analysis using 82842-7-RR (same clone as 82842-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82842-7-RR (same clone as 82842-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

• 

  ChIP experiment of K-562 using 82842-7-RR (same clone as 82842-7-PBS)

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of BACH1 (82842-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82842-7-PBS bindet in WB, IF/ICC, Indirect ELISA, ChIP-qPCR BACH1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BACH1 fusion protein Ag5128
• Vollständiger Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC063307
• Gene symbol: BACH1
• Gene ID (NCBI): 571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.