BACH1 Rekombinanter Antikörper

BACH1 Rekombinant Antikörper für WB, IF/ICC, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IF/ICC, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

4K14

Rezensionen (1)

Kat-Nr. : 82842-7-RR

Synonyme

4K14, BACH 1, BACH-1

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• 

  WB analysis using 82842-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 82842-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  ChIP experiment of K-562 using 82842-7-RR

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of BACH1 (82842-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, Hausschwein-Hirngewebe, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82842-7-RR bindet in WB, IF/ICC, ELISA, ChIP-qPCR BACH1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BACH1 fusion protein Ag5128
• Vollständiger Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC063307
• Gene symbol: BACH1
• Gene ID (NCBI): 571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Agnese (Verified Customer) (07-03-2025)

The Ab seem to work fine in our sample type (hMDM) and application (immunofluorescence and PLA).

• Applications: Immunofluorescence, Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human monocyte-derived macrophages (hMDM)