BACH2 Polyklonaler Antikörper

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• KD/KO Validated

BACH2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27635-1-AP

Synonyme

BACH2, BTB and CNC homolog 2

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• 

  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 27635-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

27635-1-AP bindet in WB, IHC, ELISA BACH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BACH2 fusion protein Ag26526
• Vollständiger Name: BTB and CNC homology 1, basic leucine zipper transcription factor 2
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_001170794
• Gene symbol: BACH2
• Gene ID (NCBI): 60468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )

Publikationen

Species	Application	Title
human	WB	FEBS Open Bio; Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.; Authors - Yujun Sheng ; KD Validated
human	WB	Cell Rep; Class I HDAC inhibitors enhance antitumor efficacy and persistence of CAR-T cells by activation of the Wnt pathway; Authors - Meng Zhu
human	WB,IHC	World J Surg Oncol; Prognostic value of circadian rhythm-associated genes in breast cancer; Authors - Ling Wang