BAG1 Polyklonaler Antikörper

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• KD/KO Validated

BAG1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 19064-1-AP

Synonyme

BAG1S/1M/1L, BAG 1, BAG-1, BAG1L speicfic, Bcl-2-associated athanogene 1

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  WB analysis of HeLa using 19064-1-AP

  WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells.

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  WB analysis of HeLa using 19064-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of UV treated Hela using 19064-1-AP

  UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19064-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 19064-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 19064-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19064-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19064-1-AP

  IP result of anti-BAG1 (IP:19064-1-AP, 4ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 1120 ug.

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  IHC staining of human testis using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 19064-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAG1 antibody (19064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 19064-1-AP

  Immunofluorescent analysis of MCF-7 cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HL-60-Zellen, HT-1080.Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

19064-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BAG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAG1 fusion protein Ag13555
• Vollständiger Name: BCL2-associated athanogene
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 30-33 kDa
• GenBank-Zugangsnummer: BC014774
• Gene symbol: BAG1
• Gene ID (NCBI): 573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

Publikationen

Species	Application	Title
		Oncol Rep; Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.; Authors - Wenbai Huang
human	IHC	Ann Rheum Dis; Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration; Authors - Yue Fan
human	WB	Int Immunopharmacol; Construction of an autophagy-related genes risk model as predicting prognosis: BAG1 suppresses growth of clear cell renal cell carcinoma; Authors - Jiayu Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart