BAG1S/1M/1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BAG1S/1M/1L Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16150-1-AP

Synonyme

BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic

---

• 

  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16150-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16150-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16150-1-AP

  IP result of anti-BAG1S/1M/1L (IP:16150-1-AP, 4ug; Detection:16150-1-AP 1:800) with Jurkat cells lysate 1320 ug.

• 

  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

16150-1-AP bindet in WB, IHC, IP, ELISA BAG1S/1M/1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: BCL2-associated athanogene
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa, 41-46 kDa, 25-33 kDa
• GenBank-Zugangsnummer: NM_004323
• Gene symbol: BAG1
• Gene ID (NCBI): 573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

Publikationen

Species	Application	Title
human	WB,IHC	Tumour Biol; Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.; Authors - Wenbai Huang ; KD Validated