BAG2 Rekombinanter Antikörper

BAG2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242717G4

Kat-Nr. : 85063-4-RR

Synonyme

242717G4, BAG-2, KIAA0576

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  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85063-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85063-4-RR against Human BAG2 were performed. The affinity constant is 0.644 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe, U-251-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85063-4-RR bindet in WB, ELISA BAG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BAG2 fusion protein Ag30873
• Vollständiger Name: BCL2-associated athanogene 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_004282
• Gene symbol: BAG2
• Gene ID (NCBI): 9532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).