BAG3 Polyklonaler Antikörper

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• KD/KO Validated

BAG3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10599-1-PBS

Synonyme

BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS, Docking protein CAIR-1

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  WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 10599-1-AP (same clone as 10599-1-PBS)

  IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10599-1-AP (same clone as 10599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10599-1-AP (same clone as 10599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10599-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA BAG3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAG3 fusion protein Ag0956
• Vollständiger Name: BCL2-associated athanogene 3
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 74-80 kDa
• GenBank-Zugangsnummer: BC006418
• Gene symbol: BAG3
• Gene ID (NCBI): 9531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.