BAG3 Monoklonaler Antikörper

BAG3 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4G6C2

Kat-Nr. : 68076-1-PBS

Synonyme

4G6C2, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS

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  WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68076-1-Ig (same clone as 68076-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using BAG3 antibody (68076-1-Ig, Clone: 4G6C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68076-1-PBS bindet in WB, IF/ICC, ELISA BAG3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAG3 fusion protein Ag17608
• Vollständiger Name: BCL2-associated athanogene 3
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC006418
• Gene symbol: BAG3
• Gene ID (NCBI): 9531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.