BAG3 Rekombinanter Antikörper

BAG3 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240860F12

Kat-Nr. : 83779-4-PBS

Synonyme

Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3

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  WB analysis using 83779-4-RR (same clone as 83779-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83779-4-RR (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83779-4-RR (same clone as 83779-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83779-4-RR (BAG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83779-4-RR (same clone as 83779-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (83779-4-RR, Clone: 240860F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83779-4-RR (same clone as 83779-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BAG3 Recombinant antibody (83779-4-RR, Clone:240860F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83779-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83779-4-RR against Human BAG3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83779-4-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA BAG3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BAG3 fusion protein Ag0956
• Vollständiger Name: BCL2-associated athanogene 3
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 74-80 kDa
• GenBank-Zugangsnummer: BC006418
• Gene symbol: BAG3
• Gene ID (NCBI): 9531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.