SODD/BAG4 Polyklonaler Antikörper

SODD/BAG4 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13913-1-AP

Synonyme

BAG 4, BAG4, Silencer of death domains, SODD

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 13913-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13913-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13913-1-AP

  IP Result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

• 

  IF Staining of MCF-7 using 13913-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13913-1-AP bindet in WB, IP, IF, ELISA SODD/BAG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Artemia sinica
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SODD/BAG4 fusion protein Ag4905
• Vollständiger Name: BCL2-associated athanogene 4
• Berechnetes Molekulargewicht: 457 aa, 50 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 66-70 kDa
• GenBank-Zugangsnummer: BC038505
• Gene symbol: BAG4
• Gene ID (NCBI): 9530
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

Publikationen

Species	Application	Title
Artemia sinica	WB	Gene; Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.; Authors - Mengchen Zhang
	WB	Nat Commun; Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila; Authors - Alexandra E. Perlegos