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  • Featured Product
  • KD/KO Validated

BAG5 Polyklonaler Antikörper

BAG5 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26628-1-AP

Synonyme

BAG 5, BAG5, KIAA0873

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of MCF-7 cells using BAG5 Polyclonal antibody (26628-1-AP)

    WB analysis of MCF-7 using 26628-1-AP

    WB result of BAG5 antibody (26628-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG5 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using BAG5 Polyclonal antibody (26628-1-AP)

    WB analysis using 26628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26628-1-AP (BAG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG5 Polyclonal antibody (26628-1-AP)

    IF Staining of MCF-7 using 26628-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26628-1-AP (BAG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, SH-SY5Y-Zellen
Erfolgreiche Detektion in IFMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

26628-1-AP bindet in WB, IF, ELISA BAG5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BAG5 fusion protein Ag24320
Vollständiger Name BCL2-associated athanogene 5
Berechnetes Molekulargewicht 51 kDa
Beobachtetes Molekulargewicht 48-51 kDa
GenBank-ZugangsnummerBC044216
Gene symbol BAG5
Gene ID (NCBI) 9529
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for BAG5 antibody 26628-1-APProtokoll herunterladen
IF protocol for BAG5 antibody 26628-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Death Discov

Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders.

Authors - Michael R Duggan
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

WEI (Verified Customer) (03-03-2022)

It have three bands, one band is missing in the KO tissue.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1: 3000
  • Cell Tissue Type: Mouse Heart