BAG6 Monoklonaler Antikörper

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• KD/KO Validated

BAG6 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B8D3

Kat-Nr. : 66661-1-Ig

Synonyme

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

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Galerie der Validierungsdaten

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  WB analysis using 66661-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 66661-1-Ig

  WB result of BAG6 antibody (66661-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG6 transfected HEK-293 cells.

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  WB analysis of HeLa using 66661-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66661-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66661-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 274.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66661-1-Ig bindet in WB, IHC, ELISA BAG6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAG6 fusion protein Ag24525
• Vollständiger Name: HLA-B associated transcript 3
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC003133
• Gene symbol: BAT3
• Gene ID (NCBI): 7917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu