BAG6 Monoklonaler Antikörper

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• KD/KO Validated

BAG6 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B8D3

Kat-Nr. : 66661-1-PBS

Synonyme

BAT3, 1B8D3, BAG 6, BAG family molecular chaperone regulator 6, BAG-6

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  WB analysis using 66661-1-Ig (same clone as 66661-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66661-1-Ig (same clone as 66661-1-PBS)

  WB result of BAG6 antibody (66661-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66661-1-Ig (same clone as 66661-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66661-1-Ig (same clone as 66661-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66661-1-Ig (same clone as 66661-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66661-1-Ig (same clone as 66661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66661-1-Ig (same clone as 66661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66661-1-Ig (same clone as 66661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66661-1-Ig (same clone as 66661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66661-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66661-1-PBS bindet in WB, IHC, Indirect ELISA BAG6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAG6 fusion protein Ag24525
• Vollständiger Name: HLA-B associated transcript 3
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC003133
• Gene symbol: BAT3/BAG-6
• Gene ID (NCBI): 7917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.