BAIAP2L1 Polyklonaler Antikörper

BAIAP2L1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 25692-1-AP

Synonyme

BAIAP2L1, IRTKS

---

• 

  WB analysis of HeLa using 25692-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25692-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 25692-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 25692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25692-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25692-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

25692-1-AP bindet in WB, IHC, ELISA BAIAP2L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAIAP2L1 fusion protein Ag22499
• Vollständiger Name: BAI1-associated protein 2-like 1
• Berechnetes Molekulargewicht: 511 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC013888
• Gene symbol: BAIAP2L1
• Gene ID (NCBI): 55971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAIAP2L1, also known as IRTKS, is a 511-amino acid protein containing an IRSp53/MIM homology domain (IMD) that bundles actin filaments and interacts with the small GTPase Rac (PMID: 17430976; 14752106). In addition to the IMD domain, BAIAP2L1 also contains an SH3 domain and WW domain interaction motif. BAIAP2L1 is widely distributed and is an INS receptor substrate. It has a role in actin remodeling and membrane protrusion.

Publikationen

Species	Application	Title
human	WB	Med Oncol; IRTKS contributes to the malignant progression of cervical cancer cells; Authors - Yan Zhang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects both human and mouse versions of the protein.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T and overexpression

BAIAP2L1 Antibody Western Blot, validation (1:1000 dilution) in 293T and overexpression (Cat no:25692-1-AP)