BAP29 Polyklonaler Antikörper

BAP29 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15796-1-PBS

Synonyme

BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29

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  WB analysis using 15796-1-AP (same clone as 15796-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 15796-1-AP (same clone as 15796-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 15796-1-AP (same clone as 15796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 15796-1-AP (same clone as 15796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15796-1-AP (same clone as 15796-1-PBS)

  Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 15796-1-AP (same clone as 15796-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug BAP29 Polyclonal antibody (15796-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15796-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA BAP29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAP29 fusion protein Ag8505
• Vollständiger Name: B-cell receptor-associated protein 29
• Berechnetes Molekulargewicht: 241 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC008478
• Gene symbol: BAP29
• Gene ID (NCBI): 55973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.